Publisher : Daya Publishing House Author : Sunita K Yadav Language : English Edition : Latest By Publishers Book Cover : Hard Back ISBN : 9789359198422
Table of Content
Acknowledgements v Preface vii The Contributors xi Abbreviations xiii General Operating Procedures 1 Safety in Laboratories 3 Part-I 1. Isolation of Genomic DNA from E. coli 7 2. Isolation of Plasmid DNA (pUC 18/19) from E. coli 17 3. To Perform Restriction Digestion of Plasmid DNA 27 4. Preparation of Competent Cells and Transformation of the Cells Using pUC18 to Calculate the Transformation Efficiency 33 5. Construction of Circular and Linear Restriction Maps from the Data Provided 43 6. To Study the Technique of DNA Fingerprinting 51 7. Study of Polymerase Chain Reaction 61 8. Western Blotting 71 9. To Demonstrate the Technique of Southern Hybridization 85 10. Northern Blotting 91 Part-II 1. Demonstration of Lymphoid Organs in Rat 99 2. Histological Study of Spleen
Thymus and Lymph Nodes through Permanent Slides/Micrographs 111 3. Preparation of a Stained Blood Film to StudyTypes of Cells 125 4. Preparation of Single Cell Suspension of Splenocytes
Cell Counting and Viability Test 139 5. To Determine the ABO Grouping of the Blood Sample 147 6. Detection of Complement Activity Using Hemolysis of Antibody Coated SRBC and Standard Serum 155 7. Enzyme-Linked Immunosorbent Assay (ELISA) 163 8. To Learn the Technique and Basic Patterns of Precipitation by Ouchterlonys Double Immuno-diffusion Method 173 9. To Learn the Technique of Immuno-electrophoresis 181 Glossary 189 Colour Plates I-VIII Index 195
