Molecular Techniques in Crop Improvement By Phundan Singh

Product details

Publisher ‏: Daya Publishing House
Author : Phundan Singh
Language ‏: English
Edition : Latest By Publishers
Book Cover : Hard Back
ISBN : 9789354610318

Table of Content

Preface vii 1. Introduction to Molecular Techniques 1 Introduction Gel Electrophoresis Polymerase Chain Reaction Gene Cloning DNA Sequencing Southern Blotting Northern Blotting Western Blotting Gene Silencing DNA Fingerprinting DNA Micro Arrays Fluorescence in situ Hybridization Gene Mapping Allele Specific Oligonucleotide Summary Questions. 2. DNA Isolation 9 Introduction Extraction of Onion DNA Extraction of Wheat Germ DNA Isolation of DNA Fragments Gene Detection Summary Questions. 3. Gene Cloning 17 Introduction Steps Involved Gene Libraries Construction of Library Advantages of Gene Cloning Limitations of Gene Cloning Application in Agriculture Summary Questions. 4. Polymerase Chain Reaction (PCR) 25 Introduction Steps Involved Applications of PCR Advantages and Disadvantages of PCR Summary Questions. 5. Gene Detection Techniques 31 IntroductionGel Electrophoresis Southern Blotting Northern Blotting Western Blotting Probes for DNA Detection Summary Questions. 6. Genotyping and Phenotyping 37 Introduction Comparison of Genotype and Phenotype Genotyping Phenotyping Comparison of Genotyping and Phenotyping Summary Questions. 7. Quantitative Trait Locus (QTL) Mapping 43 Introduction QTL Mapping Future Thrusts Summary Questions. 8. DNA Fingerprinting 49 Introduction Methods of DNA Fingerprinting Types of DNA Fingerprinting Steps Involved Advantages Drawbacks Applications in Crop Improvement Summary Questions. 9. Methods of Foreign Gene Transfer 57 Introduction Types of Gene Transfer Steps Involved Methods of Foreign Transfer Summary Questions. 10. Molecular Markers 65 Introduction Types of Marker Properties of an Ideal DNA Marker Types of DNA Markers Applications in Crop Improvement Summary Questions. 11. Marker Assisted Selection 79 Introduction Main Features Marker Assisted Selection Gene versus Marker Selection for Major Gene Linked to Marker Situation for Use of MAS Steps Involved in MAS Applications of Markers Advantages of MAS Disadvantages of MAS Summary Questions. 12. Transgenic Breeding 87 Introduction Transgenic Plants Advantages of Transgenic Technology Steps Involved Transgenic vs Conventional Breeding Applications Practical Achievements Probable Risks Future Scope Summary Questions. 13. Transgenic Male Sterility 97 Introduction Transgenic Male Sterility Antisense Gene Approach Summary Questions. 14. Mapping Populations 107 Introduction Recombinant Inbred Lines [RILS] Near Isogenic Lines [NILs] F2 Populations Backcross Populations Bulk Segregant Analysis [BSA] Doubled Haploids Summary Questions. 15. Introduction to Genomics 113 Introduction Types of Genomics Classification Proteomics Metabolomics Genomics in Crop Plants Genome Mapping in India Gene to be Mapped Some Genome Mapping Laboratories Applications in Crop Improvement Achievements Limitations Future Thrusts Summary Questions. 16. Introduction to Bioinformatics 123 Introduction Types of Bioinformatics Applications in Plant Breeding Summary Questions. 17. Gene Revolution 131 Introduction Main Features of Gene Revolution Green Revolution and Gene Revolution Advantages of GM Technology The Future of Gene Technology Summary Questions. 18. Nanotechnology in Crop Improvement 141 Introduction Main Features Brief History Applications Applications in Crop Improvement Achievements Application in Agriculture Applications in Other Fields Implications of Nanotechnology Summary Questions. 19. Reverse Breeding [RB] 151 Introduction Important Features Steps Involved Forward and Reverse Breeding Practical Applications Reverse Breeding and Marker Assisted Breeding Limitations Doubled Haploids Future Scope Summary Questions. 20. Space and Speed Breeding 159 Introduction A. Space Breeding B. Speed Breeding Summary Questions. Appendices 169 Glossary 183 Some Useful References 191 Key References 193 Books by Dr. Phundan Singh 195